| 1 | Luzia Obermeier (SLU) def. Isabela Mercante (SPC) 6-1, 6-3 |
| 2 | Lavinia Lancellotti (SLU) def. Rima Yoshida (SPC) 6-2, 7-5 |
| 3 | Alessia Ciampi (SPC) def. Arianna Bettinelli (SLU) 6-3, 6-3 |
| 4 | Maria Moskaltsova (SPC) def. Cheyenne Houkes (SLU) 1-6, 6-2, 13-11 |
| 5 | Kyoko Ueno (SPC) def. Paris Marozzelli (SLU) 5-7, 7-5, 10-8 |
| 1 | Martina Picardi (SEU) def. Nicole Dufour (SLU) 6-3, 6-3 |
| 2 | Kamilya Shailina (SLU) def. Flaminia Guidantoni (SEU) 6-4, 5-7, 10-8 |
| 3 | Simone Simas (SEU) def. Camila Keller (SLU) 6-4, 7-6 (3-0) |
| 4 | Ingrid Montserrat (SEU) def. Catherine Rabatin (SLU) 6-2, 6-2 |
| 5 | Irene Lopez Suero (SEU) def. Madelyn Martin (SLU) 6-3, 6-3 |
| 6 | Neleah Neiberline (SEU) def. Alexandra Warchausky (SLU) 6-3, 6-0 |
| 1 | Isbella Ciancaglini (STU) def. Elena Prado (EFSC) 6-2, 6-2 |
| 2 | Maria Navarro (STU) def. Emma Sanchez (EFSC) 6-3, 6-1 |
| 3 | Laura Yans (EFSC) def. Lauren Seemann (STU) 2-6, 6-2, 10-7 |
| 4 | Jenesie Burgos (STU) def. Lucia Lucas (EFSC) 1-6, 6-1, 11-9 |
| 5 | Maria Gento (EFSC) def. Lauren Fullerton (STU) 6-3, 6-1 |
| 6 | Carlota Costa Manero (STU) def. Sara Ladino (EFSC) 2-6, 6-4, 10-5 |
| 1 | Polina Vlasova (RC) def. Catherine Goldman (HCC) 6-2, 4-6, 10-6 |
| 2 | Paula Cabrer Pericas (HCC) def. Kaitlynn Groves (RC) 6-1, 6-2 |
| 3 | Jiayi Liu (RC) def. Adeola Greatorex (HCC) 2-6, 6-2, 11-9 |
| 4 | Milla Falster (RC) def. Sanne Schoorman (HCC) 6-2, 6-2 |
| 5 | Stella Fruijtier (RC) def. Rocio Azcarraga (HCC) 7-6 (0-7), 6-0 |
| 6 | Maria McKay (RC) def. Yoana Dudova (HCC) 6-4, 6-3 |
| 7 | Alicia Rodriguez (STU) def. Annie Sallick (RC) 8-2 |
| 8 | Nina Mitrofanova (RC) def. Antera Hernandez (HCC) 7-5, 6-1 |
| 9 | Aya Matsunaga (HCC) def. Eve Wasil (RC) 6-2, 6-4 |
